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Diagenics產品介紹

 更新時間:2019-07-28 點擊量:1211

Diagenics成立于1994年,憑借其在過敏診斷和治療領域多年的經驗以及用于研究和常規實驗室的ELISA試劑盒的供應和技術支持而建立了自己的名字。

Diagenics產品介紹

ELISA試劑盒和抗體

Diagenics的團隊都具有研究或體外診斷行業的背景。自成立以來,我們代表瑞典公司Mercodia在英國。我們的產品組合現已擴展到包括來自眾多精選ELISA試劑盒,抗體和相關產品的精選供應商的產品,以便我們可以為生命科學研究的幾乎所有領域的客戶提供支持。

Mercodia成立于1991年,開發,生產和銷售基于ELISA技術的診斷試劑盒,用于臨床和研究應用。

 

Mercodia ELISA試劑盒

如需訂購Mercodia產品,請聯系。

Mercodia成立于1991年,開發,生產和銷售基于ELISA技術的診斷試劑盒,用于臨床和研究應用。

臨床和研究領域

糖尿病

胰島素是負責控制葡萄糖代謝的主要激素。胰島素的分泌主要受血漿葡萄糖濃度的控制,并且激素具有許多重要的代謝作用。在胰島素依賴性糖尿?。?型糖尿?。┖鸵恍┢渌“Y如低垂體癥中胰島素濃度嚴重降低。胰島素濃度在非胰島素依賴型糖尿?。?型糖尿?。?,胰島素瘤和某些類型的內分泌功能障礙(如庫欣綜合征和肢端肥大癥)中升高。

心血管疾病

氧化低密度脂蛋白(LDL)是一種致動脈粥樣硬化斑塊特異性蛋白,直接參與動脈粥樣硬化病變的發生和發展。血管造影證實的冠狀動脈疾病患者的血漿氧化LDL水平顯著升高,現在越來越清楚氧化LDL的循環水平是冠狀動脈粥樣硬化的標志物。

載脂蛋白(a)也被研究作為動脈粥樣硬化的獨立危險因素。

ELISA分析可用于兩種分析物。

 

分析原則

ELISA序列測定和ELISA同時測定原理。

哺乳動物糖尿病

通過使用大鼠或小鼠胰島素ELISA與人胰島素ELISA(0.7%大鼠/小鼠交叉反應)的組合,可以在相同樣品中與人胰島素分開測定大鼠或小鼠胰島素濃度。大鼠胰島素ELISA,豬胰島素ELISA,牛胰島素ELISA,牛胰島素ELISA,小鼠胰島素ELISA,犬胰島素ELISA,馬胰島素ELISA和大鼠Glicentin

人類糖尿病

確定血清或細胞培養基中的胰島素,胰島素原和C-肽是尋找I型和2型糖尿病有效治療方法的有價值的工具。胰島素ELISAs,C肽ELISAs,Leptin ELISA,載脂蛋白ELISA,胰高血糖素ELISA,Glicentin ELISA,GLP-1 NL-ELISA和Lispro NL-ELISA

心血管

Mercodia提供心血管疾病檢測,重點關注動脈粥樣硬化過程的不同機制。

使用說明

PDF包含各種Mercodia產品使用說明的文件。

 

Mercodia部分產品列表

 

DescriptionRat Insulin ELISAUltrasensitive Rat Insulin ELISAHigh Range Rat Insulin ELISA
Code No.10-1250-01
10-1250-10
10-1251-01
 
10-1145-01
 
No. of tests96/10x969696
Measured antigenRat/mouse insulinRat/mouse insulinRat/mouse insulin
Standards ug/l0, 0.15, 0.4, 1, 3, 5.50, 0.02, 0.05, 0.15 0.4, 1.00, 3.0, 7.5, 30.0, 75.0, 150.0
Calibration   
Incubations (mins)120+15120+15120+15
Sample/sample volumeserum/plasma 10ulserum/plasma 25ulserum/plasma 10ul
Sensitivity≤0.15ug/l1) ≤0.02ug/l1.5 ug/l/
263 pmol/l
Precision intra/inter cv%<5.2%, <10.1%1) <3% 1) <5%<5%/<5%
Hook>450 ug/l450 ug/l576 ug/l
100.8nmol/l
Cross-reactions
  • Human insulin 167%
  • Insulin Lispro (Humalog®) 167%
  • Human proinsulin 75%
  • Human C-peptide <0.05%
  • IGF-I <0.02%
  • IGF-II <0.02%
  • Rat C-peptide <0.001%
  • Rat proinsulin 7%
  • Mouse insulin 75%
  • Porcine insulin 476%
  • Sheep insulin 179%
  • Bovine insulin 78%
  • No interference by lipaemic, icteric or haemolysed samples
  • insulin (mouse) 81%
  • insulin (human) 120%
  • insulin (Humalog®)120%
  • proinsulin (human) 71%
  • C-peptide (human) <0.05%
  • No interference by lipaemic, icteric or haemolysed samples
  • insulin (mouse) 81%
  • insulin (human) 120%
  • insulin (Humalog®) 120
  • proinsulin (human) 71% C-peptide (human)<0.05%
  • No interference by lipaemic, icteric or haemolysed samples

DescriptionPorcine Insulin ELISAOvine Insulin ELISABovine Insulin ELISA
Code No.10-1200-0110-1202-0110-1201-01
No. of tests969696
Measured antigenPorcine insulinOvine insulinBovine insulin
Standards ug/l0, 0.02, 0.05, 0.15, 0.5, 1.50, 0.05, 0.15, 0.5, 1.5, 3.00, 0.05, 0.15, 0.5, 1.5, 3.0
Calibrationin-house referencein-house referencein-house reference
Incubations (mins)120+15120+15120+15
Sample/sample volumeSerum/plasma 25ulSerum/plasma 25ulSerum/plasma 25ul
Sensitivity≤0.01ug/l≤0.025ug/l≤0.025ug/l
Precision intra/inter cv%<3.8% <3.9%<3.7% <6.5%<5.3% <7.0%
Hook>1000ug/l>1000ug/l>1000ug/l
Cross-reactionsNo interference by lipaemic, icteric or haemolysed samplesNo interference by lipaemic, icteric or haemolysed samplesNo interference by lipaemic, icteric or haemolysed samples

DescriptionMouse Insulin ELISAUltrasensitive Mouse Insulin ELISAGlucagon 10ul ELISA
Code No.10-1247-01
10-1247-10
10-1249-01
 
10-1281-01
No. of tests96/10x969696
Measured antigenMouse InsulinMouse InsulinGlucagon. validated for rat, mice, porcine & non-human primate.
Standards ug/l0, 0.188, 0.5, 1.25, 3.75, 6.90, 0.025, 0.063, 0.188, 0.5, 1.25 
Incubation (mins)120 + 15120 + 15overnight+15
Sample/sample volumeserum/plasma 10ulserum/plasma 25ulSerum/plasma/cell culture
Sensitivity≤0.2 ug/l≤0.025 ug/l1.5 pmol/l
Precision intra/inter cv%<3.2%/<6%<3%/<5%Total 8.3 17.9%
Hook>450 ug/l>450 ug/l>8 umol/l
Cross-reactionsNo interference by lipaemic, icteric or haemolysed samplesNo interference by lipaemic, icteric or haemolysed samples
  • Oxyntomodulin, human, rat, mouse 2.0%
  • Oxyntomodulin, bovine, canine, porcine n.d.
  • Mini-Glucagon n.d.
  • GLP-1 n.d.
  • GLP-2 n.d.
  • Glicentin, human 1.0%
  • Glicentin, mouse 7.0%
  • Glicentin, rat 4.0%

DescriptionRat C-peptide ELISARat/Mouse proinsulin ELISA
Code No.10-1172-0110-1232-01
No. of tests9696
Measured antigenRat C-peptideRat and Mouse proinsulin
Standards pmol/lc. 0 - 4000 (0 + 5 standards)c. 0 - 2000 (0 + 5 standards)
Incubation (mins)60 + 60 + 15120+30
Sample/sample volumeserum/plasma 10ulserum/plasma 25ul
Sensitivity%td 3 pmol/l
Precision intra/inter cv%<4.3%/<7.3%<3.7%/<6.4%
Hook400000pmol/l90000pmol/l
Cross-reactions
  • Human C-peptide <0.001%
  • Rat proinsulin 4.55%
  • Rat insulin <0.01%
  • No interference by lipaemic, icteric or haemolysed samples
  • Human insulin <0.0000001%
  • Human proinsulin 2 - 10% (mean 5%)
  • Human C-peptide <0.000001%
  • Rat C-peptide <0.0000001%
  • Rat insulin <0.000004%
  • Mouse insulin <0.0003%
  • Mouse C-peptide <0.00007%
  • No interference by lipaemic, icteric or haemolysed samples

DescriptionHigh Range Porcine Insulin ELISAFeline Insulin ELISA
Code No.10-1223-0110-1233-01
No. of tests9696
Measured antigenPorcine InsulinFeline Insulin
Standardsc. 0 - 75 g/l (0 + 5 standards)0, 5, 35, 100, 350, 700 ng/l
Incubation (mins)60 + 15120+30
Sample/sample volumeserum/plasma 10ulserum/plasma 10ul
Sensitivity0.313ug/l5ng/l
Precision intra/inter cv%<2.3%/<5%<4.8%/<8.9%
Hook11500 ug/l27600 ug/l
Cross-reactions
  • Porcine C-peptide <0.001%
  • Porcine Proinsulin <0.2%
  • Human insulin 28%
  • Human Proinsulin <0.1%
  • Human C-peptide <0.01%
  • Aspart (NovoRapid) 0.7%
  • Detemir (Levemir) <0.0000002%
  • Glargin (Lantus) 5.4%
  • Lispro (Humalog) <0.0000006%
  • No interference by lipaemic, icteric or haemolysed samples
  • Aspart (NovoRapid®) 5.9%
  • Detemir (Levemir®) <0.008%
  • Glargin (Lantus®) 8.4%
  • Lispro (Humalog®) <0.00000002%
  • Glulisine (Apidra®) <0.0000007%
  • Letsulin® , Caninsulin® 57.4%
  • No interference by lipaemic, icteric or haemolysed samples

DescriptionCanine Insulin ELISAEquine Insulin ELISA
Code No.10-1203-0110-1205-01
No. of tests9696
Measured antigenCanine InsulinEquine Insulin
Standards ug/l0, 0.02, 0.05, 0.15, 0.50, 1.500, 0.02, 0.05, 0.15, 0.50, 1.50
Calibrationin-house porcine insulin referencein-house porcine insulin reference
Incubation (mins)120 + 15120 + 15
Sample/sample volumeserum/plasma 25ulserum/plasma 25ul
Sensitivity≤0.01 ug/l≤0.01 ug/l
Precision intra/inter cv%<4.5%/<6.2%<3.4%/<4.3%
Hook>1000ug/l>1000ug/l
Cross-reactions
  • porcine insulin 100%
  • porcine C-peptide <0.001%
  • porcine proinsulin <0.20%
  • human insulin 28%
  • human C-peptide <0.01%
  • human proinsulin <0.1%
  • NovoRapid 0.7%
  • Levemir <0.0000002%
  • Lantus 5.4%
  • Humalog <0.0000006%
  • No interference by lipaemic, icteric or haemolysed samples
  • porcine insulin 100%
  • porcine C-peptide <0.001%
  • porcine proinsulin <0.20%
  • human insulin 28%
  • human C-peptide <0.01%
  • human proinsulin <0.1%
  • NovoRapid 0.7%
  • Levemir <0.0000002%
  • Lantus 5.4%
  • Humalog <0.0000006%
  • No interference by lipaemic, icteric or haemolysed samples

DescriptionPorcine C-peptide ELISARat Glicentin ELISA
Code No.10-1156-0110-1275-01
No. of tests9696
Measured antigenPorcine C-peptideRat Glicentin
Standards pmol/Lc. 0—1500 (0 + 5 standards)5—500 pmol/l
Incubation (mins)120 + 60 + 15overnight + 15
Sample/sample volumeserum/plasma/cell culture 10ulSerum/plasma 10ul
Sensitivity10 pmol/L5 pmol/L
Precision intra/inter cv%<3.7%/<3.8%Total 7—14%
Hook>30000 pmol/L>11,000 pmol/l
Cross-reactions
  • Porcine insulin <0.05%
  • Porcine proinsulin <0.02%
  • Human C-peptide <0.003%
  • Human proinsulin <0.01%
  • Macaque C-peptide <0.006%
  • Mouse C-peptide <0.007%
  • Mouse insulin <0.13%
  • Mouse proinsulin <0.002%
  • Rat C-peptide 0.08%
  • Rat insulin <0.002%
  • Rat proinsulin <0.002%
  • Oxyntomodulin ND
  • Mini-Glucagon ND
  • GLP-1 ND
  • GLP-2 ND
  • Glucagon ND
  • Human Glicentin <1.7%
  • Mouse Glicentin 5.2%

 

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